Research Profile: Identifying cell-surface glycoproteins
نویسندگان
چکیده
منابع مشابه
Murine Cell Surface Glycoproteins
Monoclonal antibodies reactive with NIH/3T3 cell surface antigens were obtained from hybridomas of murine myeloma cells fused to spleen cells of rats immunized with NIH/3T3 cell plasma membranes. Four of the antibodies, of forty that have been studied, appeared to react with allospecific antigenic determinants: they bound to NIH/3T3 cells but not to BALB/ 3T3 cells. Each of these four antibodie...
متن کاملCell surface glycoproteins of corneal epithelium.
PURPOSE To identify those plasma membrane glycoproteins of corneal epithelial cells that are synthesized in a higher amount or are downregulated during cell migration. METHODS Primary cell cultures of rabbit corneal epithelium were used. Sialic acid and terminal galactose/N-acetylgalactosamine residues of plasma membrane glycoproteins of migrating and nonmigrating corneal epithelial cells wer...
متن کاملGolgi Apparatus Production of Cell Surface Glycoproteins
For my part the present work represented the culmination of several years of graduate studies during which I was privileged to work under the supervision of Dr. Charles Philippe Leblond at McGill University. I had joined Leblond’s laboratory in 1965, just in time to receive some supervision from Marian Neutra and to witness the publication of Meutra and Leblond’s landmark paper on the uptake of...
متن کاملCell surface glycoproteins change during gastrulation in Pleurodeles waltlii.
Identification of the cell surface glycoproteins is an essential requirement for elucidating the mechanisms that mediate intercellular adhesion and cell migration in gastrulating amphibian embryos. In our experiments the glycoproteins present at the surface of isolated cells at blastula and gastrula stages were labelled with 3H-labelled sodium borohydride. The procedure included the oxidation o...
متن کاملMicrodomain Formation Controls Spatiotemporal Dynamics of Cell-Surface Glycoproteins.
The effect of galectin-mediated microdomain formation on the spatiotemporal dynamics of glycosylated membrane proteins in human microvascular endothelial cells (HMEC-1) was studied qualitatively and quantitatively by high-resolution fluorescence microscopy and artificially mimicked by metabolic glycoprotein engineering. Two types of membrane proteins, sialic acid-bearing proteins (SABPs) and mu...
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ژورنال
عنوان ژورنال: Journal of Proteome Research
سال: 2006
ISSN: 1535-3893,1535-3907
DOI: 10.1021/pr0627802